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KMID : 1156220050310010031
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Journal of Environmental Health Sciences
2005 Volume.31 No. 1 p.31 ~ p.38
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Isolation and Identification of Activated Microorganisms for Biocide Development
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Lee Jang-Hoon
Kwon Hyuk-Ku
Jung Joon-Oh
Nam Yoon-Ku
Kang Byung-Kon
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Abstract
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An anti-fungal material produced by actinomycetes was isolated from domestic soil. This actinomycetes was identified as Streptomyces albogriseus by 16S rDNA sequence. YEME (yeast extract 4 g, malt extract 10 g, glucose 4 g, D.W 1l, pH 7.00.2) medium was used for production of anti-fungal materials. S. albogriseus was cultured in a shaking incubator for 2 weeks at 150 rpm and 25℃. An anti-fungal material produced by S. albogriseus was identified at 340 nm by uv/vis- spectrometer and it showed powerful anti-fungal activity. This is the first report that secondary metabolite produced by S. albogriseus showed an activity against phytopathogenic fungi such as Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.
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